The morpho-functional characteristics of lymphoid organs and walls of the small intestine when consuming waters of various composition
Morpho-functional characteristics of lymphoid organs and walls of the small intestine when consuming water of various composition (experimental study) Golubeva I. A.
INTRODUCTION.CHAPTER 1. REVIEW OF LITERATURE HEALTH 3. RESULTS OF EFFECTS OF THE EXPOSURE TO WATER-BODY, DISTILLATED, RADON, AND DEMANIATED WATER USED IN DRINKING REGIME WITHIN 1 DUMA-KLAVA 4. RESULTS OF INVESTIGATIONS OF THE EXPOSURE TO WATER-BODY, DISTILLATED, RADON. AND DEEMANIZED WATER USED IN DRINKING REGIME WITHIN 7 DAYSKLAVA 5. RESULTS OF THE INVESTIGATIONS OF THE EXPOSURE OF WATER, DISTILLATED, RADON AND DEMAMED WATER USED IN DRINKING REGIME WITHIN 60 DAYS.
Objective: To study the nature and dynamics of structural changes in the wall of the small intestine, organs of the lymphatic system with long-term consumption of waters of different composition. Scientific novelty of the study: For the first time, a complex study of morpho-functional parameters of the small intestine wall, associated with it lymphoid formations - Peyer's plaques, as well as lymphoid organs (mesenteric and iliac lymph nodes, spleen, thymus) in response to short-term, medium duration and long-term Drinking by animals in a drinking regime of waters of different composition. The obtained data show that irrespective of the type of water in the organs under study, structural transformations take place, indicating their adaptive nature.
Changes in the structural organization of organs occur in two phases: early-voltage (1 day) and later - resistance (7 and 60 days). It is shown that by the end of the experiment, the structural and functional parameters of the lymphoid organs are not restored to the original ones. Along with this, the use of each of the waters is associated with the development of a number of structural changes specific for them in the organs studied. An obvious dependence of changes in the structural and functional organization of regional and distant lymph nodes is revealed in connection with their topographic and anatomical position, which determines the sequence of their participation in the process of "processing" of lymph flowing from the intestine and the duration of the effect. Short-term use of various waters leads to structural transformations, indicating an increase in drainage potencies of the regional lymph node and activation of lymphatic detoxication in the distant lymph node. Long-term use of different in composition water determines the structural changes in the lymphoid organs, which are associated with manifestations of immune reactions, most pronounced when using radon water. Prolonged use of distilled water contributed to the ultrastructural reorganization of endothelial cells, indicating the predominance of ultrafiltration processes over the processes of resorption. Prolonged use of radon water caused similar ultrastructural changes in endotheliocytes of both lymphatic and circulatory microvessels, characterized by a decrease in the number of organoids, which indicated a decrease in the level of plastic processes in them.
Prolonged use of de-maned water promoted the development of ultrastructure of endothelial cells of lymphatic and blood capillaries similar to those of animals that drank radon water. The most pronounced were the changes in the mitochondria (swelling), indicating their de-energized state. Provisions put on protection: 1. Prolonged use of distilled water leads to the development of morphological signs of hyperhidrosis of the small intestine wall, an increase in the size of the thymus-dependent zone in the lymph nodes, the formation of morphological signs of antigenic stimulation in Parenchyma of the thymus. 2. Prolonged use of radon water is associated with an increase in the proportion of the vascular component in the wall of the small intestine, the presence of structural signs of the predominance of mechanisms of humoral immunity in lymphoid organs. 3. Long-term use of de-watered water promotes the development of structural manifestations of hypoxia in the wall of the small intestine, the dominance of cell death processes over the regeneration processes in the lymphoid organs at early stages of the experiment.
The change in the type of drinking water was not a strong stress factor. Rather, it can be attributed to "low-intensity factors." A group of physical, chemical and other effects that have a pronounced therapeutic and bioactivation effect on the body with minimal side effects. Most relevant to the biological nature of man and close to natural in terms of intensity and quality of exposure to living organisms (Podkolzin A.A., Dontsov V.I., 1995). Long-term exposure to such weak stimuli suggests the formation of qualitatively new reactions in the interaction of the organism with exogenous cytopathic factors (Nepomnyashchikh GI, Nepomnyashchikh JI.M., 1990; Sarkisov DS, 1993). According to the results obtained, we concluded that the reaction of the small intestine wall and lymphoid organs of the animal to long-term exposure to drinking water, different in mineral composition and radon content, was subject to general biological regularities and, in our opinion, passed in two phases of the adaptive process.
The first phase of the functional stress or, according to G. Selie's theory, the alarm reaction corresponded to 1 knock of the experiment. The second stage of stabilization or resistance (stage of resistance) began with 7 days and lasted until the end of the experiment. There are 3 stages - depletion, disruption of adaptation with the phenomena of damage (stage of exhaustion) (Vogulkina VV., Kashtanova E. L., 1990), but we did not find obvious structural signs of its development in our study. There were no animal deaths, but there was also no return to the normal baseline by day 60.